FTIR microspectroscopy (FTIRM) is a promising field which characterises bone samples, as it is able to provide information on the chemical composition and structural organisation of the analysed matter without damaging it on a local level of a thin section. Introductionīone analysis methods are quite varied, ranging from the noninvasive, e.g., X-ray scanning, to the destructive, e.g., mechanical rupture tests. The developed algorithms provide the means necessary to fully compare the results between bone FTIRM studies and between different laboratories. Results and validation proved the reliability of the automatic process. Validation of the automatic method has been performed by comparison with the manual method. Furthermore, a rigorous framework has been established concerning the peak fitting method to obtain the systematic best potential solution. To solve this issue, software has been developed based on several algorithms to automate the analysis. However, spectrum analysis methods are often highly sensitive to small variations (e.g., boundary limits), thus implying a time-consuming and redundant analysis process. FTIR microspectroscopy (FTIRM) is a commonly used nondestructive method to characterise thin bone sections.
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